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bsrgi  (New England Biolabs)


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Bioz Manufacturer Symbol New England Biolabs manufactures this product  
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    New England Biolabs bsrgi
    Bsrgi, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 395 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bsrgi+hf/BsrGI-HF/bio_rxiv__64898__2026__04__21__719910-133-6-8
    Average 96 stars, based on 395 article reviews
    bsrgi - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Agarose Gel Electrophoresis:

    Article Title: Template nicking suppresses promoter-independent antisense transcription in IVT via R-loop-mediated strand displacement
    Article Snippet: .. Strand specificity was validated by digesting 200 ng of ssDNA and dsDNA with 5 U S1 nuclease in 1 × S1 buffer or 5 U BsrGI-HF® (New England Biolabs, #R3575S) in NEB rCutSmartTM Buffer at 37°C for 20 min. Products were heat-inactivated at 80°C for 20 min and analyzed by 1% agarose gel electrophoresis (GelRed-stained) alongside the 100 bp DNA Ladder (New England Biolabs, #N0467S). .. Partially duplex templates were prepared by annealing the ssDNA with complementary 80 nt oligonucleotide encoding a T7 promoter ( ) and analyzed by 4% native polyacrylamide gel electrophoresis (PAGE) with SYBRTM Gold (Invitrogen, #S11494) at 40 V for 2 h.

    Amplification:

    Article Title: Single-cell CRISPR activation screens in primary B cells discover gene regulatory mechanisms for hundreds of autoimmune risk loci
    Article Snippet: Peripheral blood mononuclear cells were isolated by spinning this mixture for 30 min at 800 × g with minimal break and acceleration, before enrichment of CD19 + B cells by positive selection with CD19 MicroBeads (Miltenyi Biotec) following the manufacturer’s protocol. .. The blasticidin resistance gene in LentiSAMv2 (a gift from Feng Zhang; Addgene #75112 ( )) was replaced with Cherry Fluorescent Protein (ChFP) by digesting LentiSAMv2 with BsrGI-HF (New England Biolabs, NEB) and EcoRI-HF (NEB) and replaced with the ChFP gene amplified from pLVX-EF1α-IRES-mCherry vector (Takara Bio 631987) to generate LentiSAMv2-ChFP . .. The subsequent dCas9-VP64-T2A-ChFP fragment was amplified and cloned into retroviral vector MSCV-BCL6-t2A-BCL2 (Addgene #135305; ( , )) digested with XhoI (NEB) and SalI-HF (NEB) to replace the BCL6-T2A-BCL2-IRES-CD2 fragment and generate MSCV-dCas9-VP64-ChFP .

    other:

    Article Title: Mapping high resolution, multidimensional phase diagrams of physiological protein condensates
    Article Snippet: Each amplicon was cloned into the bacterial expression vector pOPINS (Merck) containing a ULP protease cleavable N-terminal His-SUMO Tag which was linearised using primers SQ_005-006.




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